DNA Replication and Bacteria Lab

1. Helicase unzip DNA

2. Primase reads from 3' to 5' and creates short RNA primers

3. DNA Polymerase III form DNA complementary pairs from 3' to 5' (DNA doesn't bond with RNA primers)

4. Primase jumps backwards from 5' to 3' to the closest T or A and build RNA primers from 3' to 5'

5. DNA filled in by Polymerase III
6. DNA Polymerase I changes RNA to DNA
7. Ligase creates phosphodiester bonds between DNA fragments (Okazake fragments)

Update on the neon bacteria lab:

Bacteria sample with plasmid DNA that allow bacteria to glow and immune to antibodies in agar plate with ampicillin and arabinose: bacteria grew out and glowed.

Bacteria sample with plasmid DNA that allow bacteria to glow and immune to antibodies in agar plate with ampicillin but without arabinose: Grew out but did not glow.

Bacteria sample without plasmid DNA that allow bacteria to glow and immune to antibodies in agar plate with ampicillin: Did not grow out.

Bacteria sample without plasmid DNA that allow bacteria to glow and immune to antibodies in agar plate without ampicillin: Grew out.